Publication Highlight: Targeted deep sequencing of mycobacteria species from extrapulmonary sites not identified by routine line probe assays
In a recent study GenPath Africa consortium members Wynand Goosen, Michele Miller, Rob Warren and Tanya Kerr from Stellenbosch University (SU) used advanced sequencing techniques to investigate previously unidentified mycobacteria species in TB-infected sites outside the lungs.
What is this publication about?
This study investigates previously unidentified nontuberculous mycobacteria (NTM) species in samples taken from TB-infected sites outside the lungs. Researchers analysed 28 mycobacterial cultures using advanced sequencing techniques, including targeted next-generation sequencing, to accurately identify mycobacterial infections. Remarkably, 68% of the cultures contained mixtures of different mycobacterial species. Researchers also found TB-NTM co-infections in samples that traditional diagnostic methods previously identified as Mycobacterium tuberculosis complex negative.
Why is this important?
This publication highlights the growing recognition of NTM as significant opportunistic pathogens, especially as traditional diagnostic methods often overlook mixed infections. The study demonstrates the capabilities of next-generation sequencing in identifying complex mycobacterial profiles, which is crucial for patient management.
How can it make a difference?
By advocating for the integration of next-generation sequencing into diagnostic protocols, this research offers the potential for more accurate identification of NTM infections and can lead to better informed treatment strategies, thus ultimately addressing a global health challenge.
Christoffel Opperman, Janré Steyn, Megan Ceris Matthews, Sarishna Singh, Yonas Ghebrekristos, Tanya Jane Kerr, Michele Miller, Aliasgar Esmail, Helen Cox, Robin Warren, Giovanni Ghielmetti, Wynand J. Goosen. "Targeted deep sequencing of mycobacteria species from extrapulmonary sites not identified by routine line probe assays: a retrospective laboratory analysis of stored clinical cultures." IJID Regions, Volume 13, 100464, December 2024 (available online 24 September 2024). https://doi.org/10.1016/j.ijregi.2024.100464
